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Mass spectrometry: Come of age for structural and dynamical biology

Abstract:
Over the past two decades, mass spectrometry (MS) has emerged as a bone fide approach for structural biology. MS can inform on all levels of protein organization, and enables quantitative assessments of their intrinsic dynamics. The key advantages of MS are that it is a sensitive, high-resolution separation technique with wide applicability, and thereby allows the interrogation of transient protein assemblies in the context of complex mixtures. Here we describe how molecular-level information is derived from MS experiments, and how it can be combined with spatial and dynamical restraints obtained from other structural biology approaches to allow hybrid studies of protein architecture and movements. © 2011 Elsevier Ltd.

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Publisher copy:
10.1016/j.sbi.2011.08.002

Authors



Journal:
Current Opinion in Structural Biology More from this journal
Volume:
21
Issue:
5
Pages:
641-649
Publication date:
2011-10-01
DOI:
EISSN:
1879-033X
ISSN:
0959-440X


Language:
English
Pubs id:
pubs:192917
UUID:
uuid:03021ddf-5a87-4502-8ec3-c8590357305f
Local pid:
pubs:192917
Source identifiers:
192917
Deposit date:
2012-12-19

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